Gene Name Synonym

IFN-gamma, IFNγ, Interferon-gamma, Interferon-γ

Species

Human

Accession

P01579

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

Gln24-Gly161 is expressed with a 10×His tag at the C terminus

Molecular Weight

16.2 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and   mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA   for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution,   it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier   protein). It is recommended to freeze aliquots at -20°C or -80°C for extended   storage.

BACKGROUND

IFN gamma, also known as IFNG, is a secreted protein that belongs to   the type II interferon family. The IFNG monomer consists of a core of six   α-helices and an extended unfolded sequence in the C-terminal region.   Interferon (IFN)-γ is the uppermost cytokine implicated in anti-tumor   immunity. With its cytostatic, pro-apoptotic and immune-provoking effects,   IFN-γ plays a central role in the recognition and elimination of transformed   cells. Interferon-γ (IFN-γ) also plays a key role in activation of cellular immunity   and subsequently, stimulation of antitumor immune-response.IFN-γ is   considered potentially useful for adjuvant immunotherapy for different types   of cancer.IFN-γ is considered potentially useful for adjuvant immunotherapy   for different types of cancer. The importance of IFN-γ in the immune   system stems in part from its ability to inhibit viral replication directly,   and most importantly from its immunostimulatory and immunomodulatory effects.

Data

SDS-PAGE

Bioactivity

Active Recombinant Human IFN-gamma Protein was determined by   SDS-PAGE with Coomassie Blue, showing a band at 18-30 kDa.

Determined by its ability to induce STAT1/STAT2 activation in Human   COLO 205 ISRE LUC reporter cells.The ED50 for this effect is 1-5 ng/mL.