Gene Name Synonym

HBGF-2; FGF-2; FGF-b; FGF-basic; bFGF

Species

Rat

Accession

P13109

Source

E.coli

SPECIFICATIONS

Amino acid sequence:

E.coli-derived Rat bFGF protein, Pro 10-Ser 154.

Molecular Weight:

Approximately 16.4 kDa.

Purity:

>97.0% as determined by SDS-PAGE

Endotoxin:

<1.0 EU/μg of the protein by the LAL method.

Formulation:

Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution:

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

Basic Fibroblast Growth Factor (bFGF, also known as FGF2 and HBGF-2), encoded by the FGF2 gene, is a member of the fibroblast growth factor (FGF) family involved in bone healing, cartilage repair, bone repair, and nerve regeneration. bFGF is also a mitotic promoter that accelerates cell proliferation. bFGF regulates immune processes by specifically targeting tyrosine kinase receptors and activating the FGF/FGFR signaling pathway. bFGF combined with FGFR1/3 promoted degeneration and repair of articular cartilage, respectively. bFGF is also a known carcinogen in GBM, which contributes to glioma growth and vascularization. bFGF plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. bFGF are also involved in a variety of biological processes, including embryonic development, morphogenesis, tissue repair, tumor growth and invasion. Additionally, bFGF is frequently used for a critical component of cell culture medium, e.g., human embryonic stem cell culture medium, serum-free culture systems.

Data

SDS-PAGE

Bioactivity

Recombinant Rat bFGF Protein was determined by SDS-PAGE with Coomassie Blue.

Measured in a cell proliferation assay using Balb/c 3T3 A31 mouse embryonic fibroblasts.  The ED50 for this effect is typically 1-15 ng/mL.