Gene Name   Synonym

FSP, GRO1, GROa, MGSA, MGSA-a, NAP-3, SCYB1, CXCL1

Species

Mouse

Accession

P12850

Source

HEK293   cells

SPECIFICATIONS

Amino acid   sequence

Ala25-Lys96   is expressed with a 10×His tag at the C terminus

Molecular   Weight

7.8 kDa

Purity

>95.0%   as determined by SDS-PAGE

Endotoxin

<5 EU   mg^-1

Formulation

Lyophilized   from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as   protectants before lyophilization.

Reconstitution

Dissolved   in sterile water, it is recommended to add 0.1% HSA or BSA for long-term   preservation.

STORAGE

Stability   and Storage

12 months   from date of receipt at -20°C to -70°C. After reconstitution, it is stable at   4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is   recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

Chemokine   (C-X-C motif) ligand 1 (CXCL1) is a small molecule of cytokines belonging to   the CXC chemokine family. Also known as growth-regulated oncogene alpha   (Growth-regulated oncogene alpha, GROα). Its also known as   keratinocytes-derived chemokine (KC) in mice or cytokine-induced neutrophil   chemoattractant type-1 (CINC-1) in rats. In humans, this protein is encoded   by the gene Cxcl1 and is located on human chromosome 4 among genes for other   CXC chemokines. The chemokine CXCL1 is expressed by macrophages, neutrophils   and epithelial cells. The chemokine CXCL1 has a chemotactic effect on   neutrophils. CXCL1 binds to the chemokine receptor CXCR2 and plays a role in   cell chemotaxis. Human CXCL1 is clustered on chromosome 4 adjacent to many   CXC chemokine genes. Major roles of CXCL1 include neovascularization,   inflammation, wound healing, and tumorigenesis.

Data

SDS-PAGE

Bioactivity

Recombinant   Mouse CXCL1 Protein was determined by SDS-PAGE with Coomassie Blue.

Measured   in a cell proliferation assay using human umbilical vein endothelial cells.   The ED50 for this effect is 50-100 ng/mL.