Gene Name Synonym

HBEGF, heparin-binding EGF-like, Growth Factor, DTR, DTS, HEGFL

Species

Human

Accession

Q99075

Source

E.coli

SPECIFICATIONS

Amino acid sequence

E.coli-derived Human HB-EGF protein, Asp63-Leu148.

Molecular Weight

Approximately 10.0 kDa.

Purity

>97.0% as determined by SDS-PAGE.

Endotoxin

<1.0 EU/μg of the protein by the LAL method.

Formulation

Lyophilized from sterile Tris-Hcl, pH 8.0. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

Heparin-binding epidermal growth factor-like growth factor (HBEGF) is a heparin-binding member of the EGF family. It is a potent mitogen and chemotactic factor for fibroblasts and smooth muscle cells. As with other EGF family members, HB-EGF binds and activates EGF receptors 1 and 4. HB-EGF has been shown to stimulate the growth of a variety of cells in an autocrine or paracrine manner and to be involved in stromal proliferation. HB-EGF is also a potent inducer of tumor growth and angiogenesis. HB-EGF is found in cerebral neurons. Its expression is increased after hypoxic or ischemic injury, which also stimulates neurogenesis. HB-EGF has been implicated as a participant in a variety of normal physiological processes such as blastocyst implantation, wound healing, and in pathological processes such as tumor growth, SMC hyperplasia and atherosclerosis. HB-EGF is an 87 amino acid mitogenic and chemotactic glycoprotein containing an EGF-like domain with six conserved cysteine residues. Human HB-EGF shares about 73% and 76% aa. sequence identity with murine and rat HB-EGF.

Data

SDS-PAGE

Bioactivity

Recombinant Human HB-EGF Protein was determined by SDS-PAGE with Coomassie Blue.

Measured in a cell proliferation assay using Balb/C 3T3 A31 mouse embryonic fibroblast cells. The ED50 for this effect is 0.1-1 ng/mL.