Gene Name Synonym

PDL1, CD274, B7H1, PDCD1L1, PDCD1LG1

Species

Human

Accession

Q9NZQ7

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

>sp|Q9NZQ7|19-238 is expressed with a 10×His tag at the N terminus

Molecular Weight

25.2 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

PD-L1, which we first identified as a homologue of B7 family molecules, is expressed on immune cells, i.e., dendritic cells (DCs) and B and T cells, and controls T-cell immune responses such as peripheral tolerance, termination of immune responses and immune exhaustion after prolonged exposure to an antigen stimulus. PD-L1 plays a crucial role in the disease progression of multiple myeloma. PD-L1 has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1 can bind to PD-1 expressed on cytotoxic T lymphocytes (CTLs), resulting in the escape from immune surveillance by the induction of CTL apoptosis. PD-L1 is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.

Data

SDS-PAGE

Bioactivity

Active Recombinant Human PDL1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 30-40 kDa.

Measured by its binding ability in a functional ELISA. Immobilized human PDL1 at 3 μg/ml (100 μl /well) can bind biotinylated human PD1 with a linear range of 0.039-0.652 μg/mL.