Gene Name   Synonym

Tumor   Necrosis   Factor, TNFA, TNFSF2, Cachectin, Cytotoxin, Differentiation-inducing   factor, DIF.

Species

Mouse

Accession

P06804

Source

E.coli

SPECIFICATIONS

Amino acid   sequence

E.coli-derived Mouse TNFα   protein, Leu80-Leu235.

Molecular   Weight

Approximately   17.0 kDa.

Purity

>98.0%   as determined by SDS-PAGE.

Endotoxin

<1.0 EU/μg   of the protein by the LAL method.

Formulation 

Lyophilized   from sterile Tris-Hcl, pH 8.0. Normally 5%-8% trehalose and mannitol are   added as protectants before lyophilization.

Reconstitution

Dissolved   in sterile water, it is recommended to add 0.1% HSA or BSA for long-term   preservation.

STORAGE

Stability and Storage

12 months   from date of receipt at -20°C to -70°C. After reconstitution, it is stable at   4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is   recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

Tumour   Necrosis Factor alpha (TNF alpha) is a potent pro-inflammatory   cytokine, also known as cachectin, is a polypeptide   cytokine produced by monocytes and macrophages. TNF alpha exists in two   forms; a type II transmembrane protein (tmTNF-α) and a mature soluble protein   (sTNF-α). TNF alpha binds to its receptors, mainly TNFR1 and TNFR2, and then   transmits molecular signals for biological functions such as inflammation and   cell death. Both sTNF-α and tmTNF-α activate TNFR1, and process a   death domain (DD) that interacts with the TNFR1-associated death domain   (TRADD) adaptor protein. The TNFR2 signaling pathway is mainly activated by   tmTNF-α. TNFα receptor signaling can simultaneously activate caspase 8, the   transcription factor, NF-κB and the kinase, JNK. TNF alpha stimulates   NF-κB pathway via TNFR2 promotes cancer growth, invasion, and metastasis.The   amino acid sequence of human TNF alpha protein has low homology between   mouse, rat, bovine TNF alpha protein. While, human TNF alpha shares 94.85% aa   sequence identity with cynomolgus TNF alpha protein, mouse TNF alpha shares   94.47% aa sequence identity with rat TNF alpha protein.

Data

SDS-PAGE

Bioactivity

Recombinant   Mouse TNFα Protein was determined by SDS-PAGE with Coomassie   Blue.

Measured   in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence   of the metabolic inhibitor actinomycin D. The ED50 for this effect is   typically 1-30 pg/mL.