Gene Name Synonym

IGF-2, Somatamedin A, IGF-II

Species

Human

Accession

P01344

Source

E.coli

SPECIFICATIONS

Amino acid sequence

E.coli-derived

Human IGF-2 protein, Ala25-Glu91.

Molecular Weight

Approximately 7.5 kDa.

Purity

>97.0% as determined by SDS-PAGE.

Endotoxin

<1.0 EU/μg of the protein by the LAL method.

Formulation

Lyophilized from sterile Tris-Hcl, pH 8.0. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

Insulin-like growth factor 2 (IGF-2/IGF-II) is a member of the insulin family of polypeptide growth factors, which are involved in development and growth. Both IGF-I and IGF-II are expressed in many tissues and cell types and may have autocrine, paracrine and endocrine functions, and its mitogenic effects are mediated by their binding to the IGF plasma membrane receptors. It binds to at least two different types of receptors: IGF type 1 (IGF 1R) and mannose 6-phosphate/IGF type 2 (M6-P/IGF 2R).The IGF 1R binding to both IGF-I and IGF-II, is thought to be the predominate receptor involved in mediating the effects of these growth factors in most cell types, including osteoblasts. IGF-2/IGF-II is a mediator of prolactin-induced alveologenesis; prolactin, IGF-2, and cyclin D1, all of which are overexpressed in breast cancers, are components of a developmental pathway in the mammary gland. IGF-2 and exhibited statistically significant positive associations with colorectal cancer risk. Circulating IGF-2 and IGFBP-3 can serve as early indicators of impending colorectal cancer. IGF-2/IGF-II appears to be involved in the progression of many tumors.

Data

SDS-PAGE

Bioactivity

Recombinant Human IGF-2 Protein was determined by SDS-PAGE with Coomassie Blue.

Measured in a cell proliferation assay using PDC-P1 cells. The ED50 for this effect is 0.01-0.1 μg/mL.