Gene Name Synonym

Tumor Necrosis   Factor, TNFA, TNFSF2, Cachectin, Cytotoxin, Differentiation-inducing   factor, DIF

Species

Human

Accession

P01375

Source

E.coli

SPECIFICATIONS

Amino acid sequence

E.coli-derived Human TNFα protein,Val77-Leu233.

Molecular Weight

Approximately 17.0 kDa.

Purity

>98.0% as determined by SDS-PAGE.

Endotoxin

<1.0 EU/μg of the protein by the LAL   method.

Formulation

Lyophilized from sterile Tris-Hcl, pH 8.0. Normally 5%-8% trehalose and   mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA   for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution,   it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier   protein). It is recommended to freeze aliquots at -20°C or -80°C for extended   storage.

BACKGROUND

Tumour Necrosis Factor alpha (TNF alpha) is a potent pro-inflammatory   cytokine, also known as cachectin, is a polypeptide   cytokine produced by monocytes and macrophages. TNF alpha exists in two   forms; a type II transmembrane protein (tmTNF-α) and a mature soluble protein   (sTNF-α). TNF alpha binds to its receptors, mainly TNFR1 and TNFR2, and then   transmits molecular signals for biological functions such as inflammation and   cell death. Both sTNF-α and tmTNF-α activate TNFR1, and process a   death domain (DD) that interacts with the TNFR1-associated death domain   (TRADD) adaptor protein. The TNFR2 signaling pathway is mainly activated by   tmTNF-α. TNFα receptor signaling can simultaneously activate caspase 8, the   transcription factor, NF-κB and the kinase, JNK. TNF alpha stimulates   NF-κB pathway via TNFR2 promotes cancer growth, invasion, and metastasis.The   amino acid sequence of human TNF alpha protein has low homology between   mouse, rat, bovine TNF alpha protein. While, human TNF alpha shares 94.85% aa   sequence identity with cynomolgus TNF alpha protein, mouse TNF alpha shares   94.47% aa sequence identity with rat TNF alpha protein.

Data

SDS-PAGE

Bioactivity

Recombinant Human TNFα Protein was determined   by SDS-PAGE with Coomassie Blue.

Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in   the presence of the metabolic inhibitor actinomycin D. The ED50 for this   effect is typically 0.03-0.15 ng/mL.