Gene Name Synonym

TNF-alpha, TNFα, DIF, TNFA, TNFSF2, cachexin, cachectin

Species

Human

Accession

P01375

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

Val77-Leu233 is expressed with a 10×His tag at the N terminus

Molecular Weight

18.8 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and   mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA   for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution,   it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier   protein). It is recommended to freeze aliquots at -20°C or -80°C for extended   storage.

BACKGROUND

Tumor necrosis factor alpha (TNF-alpha ), also known as cachectin and   TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic   molecule that plays a central role in cell proliferation, differentiation,   apoptosis, lipid metabolism, and coagulation. TNF-alpha binds the ubiquitous   55-60 kDa TNF RI and the hematopoietic cell-restricted 80 kDa TNF RII, both   of which are also expressed as homotrimers. Both type I and type II receptors   bind TNF-alpha with comparable affinity, although only TNF RI contains a   cytoplasmic death domain which triggers the activation of apoptosis. Soluble   forms of both types of receptors are released and can neutralize the   biological activity of TNF-alpha. TNF-alpha protein is produced mainly by   macrophages, and large amounts of this cytokine are released in response to   lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1).   TNF-alpha is involved in fighting against the tumorigenesis, thus, is   regarded as a molecular insight in cancer treatment.

Data

SDS-PAGE

Bioactivity

Active Recombinant Human TNF-alpha Protein was determined by   SDS-PAGE with Coomassie Blue.