Gene Name Synonym

RP1-261G23.1, MGC70609, MVCD1, VEGFA, VPF

Species

Mouse

Accession

Q00731

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

Ala27-Arg214 is expressed with a 8×His tag at the C terminus

Molecular Weight

23.2 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and   mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA   for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution,   it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier   protein). It is recommended to freeze aliquots at -20°C or -80°C for extended   storage.

BACKGROUND

Vascular endothelial growth factor A (VEGFA),also known as Vascular   permeability factor (VPF). VEGFA belongs to the PDGF/VEGF growth factor   family. VEGFA is a glycosylated mitogen that specifically acts on endothelial   cells and has various effects, including mediating increased vascular   permeability, inducing angiogenesis, vasculogenesis and endothelial cell   growth, promoting cell migration, and inhibiting apoptosis. Alternatively   spliced transcript variants, encoding either freely secreted or cell-associated   isoforms, have been characterized. VEGFA is produced by a group of three   major isoforms as a result of alternative splicing and if any three isoforms   are produced (VEGFA120, VEGFA164, and VEGFA188) then this will not result in   vessel defects and death of the full VEGFA knockout in mice.

Data

SDS-PAGE

Bioactivity

Active Recombinant Mouse VEGFA Protein was determined by   SDS-PAGE with Coomassie Blue.

Measured in a cell proliferation assay using human umbilical vein   endothelial cells (HUVEC-T1). The ED50 for this effect is typically 0.5-1   μg/mL.