Gene Name Synonym

IGF-I, IGF1A, somatomedin C, MGF

Species

Human

Accession

P49764

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

Gly49-Ala118 is expressed with a 10×His tag at the C terminus

Molecular Weight

9.1 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and   mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA   for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution,   it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier   protein). It is recommended to freeze aliquots at -20°C or -80°C for extended   storage.

BACKGROUND

Insulin-like growth factor 1 (IGF-1) is also known as somatomedin C,   IGF1A, IGFI, sulfation factor, and is a hormone similar in molecular   structure to insulin. It plays an important role in childhood growth and   continues to have anabolic effects in adults. A synthetic analog of IGF-1,   mecasermin is used for the treatment of growth failure. IGF-1 consists of 70   amino acids in a single chain with three intramolecular disulfide bridges.   IGF-1 has a molecular weight of 7649 daltons. IGF-1 is produced primarily by   the liver as an endocrine hormone as well as in target tissues in a   paracrine/autocrine fashion. IGF-1 binds to at least two cell surface   receptors: the Insulin-like growth factor 1 receptor, abbreviated as   "IGF1R", and the insulin receptor. The IGF-1 receptor seems to be   the "physiologic" receptor - it binds IGF-1 at significantly higher   affinity than the IGF-1 that is bound to the insulin receptor. Like the   insulin receptor, the IGF-1 receptor is a receptor tyrosine kinase - meaning   it signals by causing the addition of a phosphate molecule on particular   tyrosines. Its primary action is mediated by binding to its specific receptor   IGF1R, present on many cell types in many tissues. Binding to the IGF1R, a   receptor tyrosine kinase, initiates intracellular signaling; IGF-1 is one of   the most potent natural activators of the AKT signaling pathway, a stimulator   of cell growth and proliferation, and a potent inhibitor of programmed cell   death.

Data

SDS-PAGE

Bioactivity

Active Recombinant Human IGF1 Protein was determined by SDS-PAGE   with Coomassie Blue.

Measured in a cell proliferation assay using PDC-P1 cells. The ED50 for   this effect is 0.05-0.1 μg/mL.