Gene Name Synonym

PGF, PLGF, PlGF, PGFL, SHGC-10760

Species

Mouse

Accession

P49764

Source

CHO Cells

SPECIFICATIONS

Amino acid sequence

Gly19-Pro158 is expressed with a 10×His tag at the C terminus

Molecular Weight

20.3 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU·mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and   mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA   for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution,   it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier   protein). It is recommended to freeze aliquots at -20°C or -80°C for extended   storage.

BACKGROUND

Placental growth factor (PGF) is also known as vascular endothelial   growth factor-related protein, PLGF and PlGF2, is a member of the VEGF   (vascular endothelial growth factor) sub-family - a key molecule in   angiogenesis and vasculogenesis, in particular during embryogenesis. The main   source of PGF during pregnancy is the placental trophoblast. PGF is also   expressed in many other tissues, including the villous trophoblast. PGF is   actived in angiogenesis and endothelial cell growth, stimulating their proliferation   and migration. PlGF2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a   heparin-dependent manner. Also promotes cell tumor growth.

Data

SDS-PAGE

Bioactivity

Testing in progress

Recombinant Mouse PGF Protein was determined by SDS-PAGE with   Coomassie Blue, showing a band at 25-35 kDa.