Gene Name   Synonym

LIF, CDF, DIA, HILDA, MLPLI

Species

Mouse

Accession

P09056

Source

HEK293   cells

SPECIFICATIONS

Amino acid   sequence

Ser24-Phe203   is expressed with a 10×His tag at the N terminus

Molecular   Weight

21.3 kDa（The protein migrates as 35-55 kDa   under reducing condition (SDS-PAGE) due to glycosylation）

Purity

>95.0%   as determined by SDS-PAGE

Endotoxin

<5 EU   mg^-1

Formulation

Lyophilized   from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as   protectants before lyophilization.

Reconstitution

Dissolved   in sterile water, it is recommended to add 0.1% HSA or BSA for long-term   preservation.

STORAGE

Stability and Storage

12 months   from date of receipt at -20°C to -70°C. After reconstitution, it is stable at   4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is   recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

Leukemia   inhibitory factor (LIF) is the most pleiotropic member of the   interleukin-6 family of cytokines. It utilises a receptor that consists   of the LIF receptor β and gp130 and this receptor complex is also used   by ciliary neurotrophic growth factor (CNTF), oncostatin M,   cardiotrophin1 (CT1) and cardiotrophin-like cytokine (CLC). This leads   to the activation of the JAK/STAT and MAPK cascades. LIF does appear to   have non-redundant actions in maternal receptivity to blastocyst implantation,   placental formation and in the development of the nervous system. LIF is   involved in growth promotion and cell differentiation of different   types of target cells, influence bone metabolism, cachexia, neural   development, embryogenesis, and inflammation. LIF is involved in the   pathogenic events and development of many diseases of various origins.

Data

SDS-PAGE

Bioactivity

Testing in   progress

Recombinant   Mouse LIF Protein was determined by SDS-PAGE with Coomassie Blue,   showing a band at 35-50 kDa.