Gene Name   Synonym

IL-4, IL4, BCGF1, BSF1

Species

Human

Accession

P05112

Source

HEK293   cells

SPECIFICATIONS

Amino acid   sequence

His25-Ser153

Molecular Weight

15 kDa

Purity

>95.0%   as determined by SDS-PAGE

Endotoxin

<5 EU   mg^-1

Formulation

Lyophilized   from sterile PBS, pH 7.4. Normally 5%-8% trehalose and mannitol are   added as protectants before lyophilization.

Reconstitution

Dissolved   in sterile water, it is recommended to add 0.1% HSA or BSA for long-term   preservation.

STORAGE

Stability and Storage

12 months   from date of receipt at -20°C to -70°C. After reconstitution, it is stable at   4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is   recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

Interleukin-4,   also known as IL4, is a secreted protein that belongs to the IL-4 /   IL-13 family. IL-4 was first identified as a T cell-derived growth   factor for B cells. In addition to T cells, IL-4 is produced by   innate lymphocytes, such as NTK cells, and myeloid cells, such as   basophils and mast cells. Interleukin-4 / IL4 has many biological roles,   including the stimulation of activated B-cell and T-cell   proliferation. Interleukin-4 / IL4 also regulates the expression of   the low-affinity Fc receptor for IgE (CD23) on both lymphocytes and   monocytes. Interleukin-4 is essential for the switching of B cells to   IgE antibody production and the maturation of T helper (Th) cells toward   the Th2 phenotype. It participates in at least several B-cell   activation processes as well as other cell types.

Data

SDS-PAGE

Bioactivity

Active   Recombinant Human IL-4 Protein was determined by SDS-PAGE with Coomassie   Blue.