Gene Name Synonym

IL-33, IL33, C9orf26, IL1F11, NFHEV

Species

Human

Accession

O95760

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

His109-Thr270 is expressed with a 10×His tag at the C terminus

Molecular Weight

19.7 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU·mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

Interleukin 33 (IL-33), is a pro-inflammatory protein and a chromatin-associated cytokine of the IL-1 family with high sequence and structural similarity to IL-1 and IL-18. IL-33 protein is expressed highly and rather selectively by high endothelial venule endothelial cells (HEVECs) in human tonsils, Peyer's patches, and lymph nodes. IL-33 protein has transcriptional regulatory properties, and the researches suggested that IL-33 is a dual-function protein that might act both as a cytokine and as an intracellular nuclear factor. As a type 2 cytokines, IL-33 protein also play a pivotal role in helminthic infection and allergic disorders.

Data

SDS-PAGE

Bioactivity

Recombinant Human IL-33 Protein was determined by SDS-PAGE with Coomassie Blue.

Determined by its ability to induce NF-κb/AP-1 activation in U251 NF-κb/AP-1 LUC reporter cells.The ED50 for this effect is 10-50 ng/mL.