Synonym

IL12B,p40,CLMF,CLMF2,NKSF,NKSF2

Species

Human

Accession

P29460

Source

HEK293   cells

SPECIFICATIONS

Amino acid   sequence

Ile23-Ser328 is   expressed with a 10×His tag at the C terminus

Molecular   Weight

36.1 kDa

Purity

>95.0%   as determined by SDS-PAGE

Endotoxin

<5 EU·mg^-1

Formulation

Lyophilized   from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants   before lyophilization.

Reconstitution

Dissolved   in sterile water, it is recommended to add 0.1% HSA or BSA for long-term   preservation.

STORAGE

Stability and Storage

12 months   from date of receipt at -20°C to -70°C. After reconstitution, it is stable at   4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is   recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

Subunit   beta of interleukin 12 (IL12B) is a subunit of human interleukin   12. Cytokine that can act as a growth factor for activated T and NK   cells, enhance the lytic activity of NK/lymphokine-activated killer cells,   and stimulate the production of IFN-gamma by resting PBMC. Interleukin   12 is a disulfide-linked heterodimer composed of the 40 kD cytokine   receptor-like subunit encoded by this gene, and a 35 kD subunit encoded by   IL12A. IL12B/IL-12B is expressed by activated macrophages that serve as an   essential inducer of Th1 cells development. This cytokine is important for   sustaining a sufficient number of memory/effector Th1 cells to mediate   long-term protection to an intracellular pathogen. IL12B Associates with   IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions   in innate and adaptive immunity.

Data

SDS-PAGE

Bioactivity

Active   Recombinant Human IL-12 beta Protein was determined by   SDS-PAGE with Coomassie Blue.