Gene Name Synonym

IL-10, Il10, Il-10

Species

Rat

Accession

P29456

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

Met19-Asn178 is expressed with a 6×His tag at the C terminus

Molecular Weight

21.6 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and   mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA   for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution,   it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier   protein). It is recommended to freeze aliquots at -20°C or -80°C for extended   storage.

BACKGROUND

Interleukin-10 (IL-10) is an anti-inflammatory cytokine that belongs to   the IL-10 family.The Interleukin (IL)-10 cytokine family includes IL-10,   IL-19, IL-20, IL-22, IL-24, and IL-26, which are considered as Class   2α-helical cytokines. IL-10, a cytokine with anti-inflammatory   properties, has a central role in infection by limiting the immune response   to pathogens and thereby preventing damage to the host. IL10 is produced by a   variety of cell lines, including T-cells, macrophages, mast cells, and other   cell types, while it is produced primarily by monocytes and to a lesser   extent by lymphocytes.

Data

SDS-PAGE

Bioactivity

Active Recombinant Rat IL-10 Protein was determined by SDS-PAGE with   Coomassie Blue.

Measured in a cell proliferation assay using MC/9 mouse mast cells. The   ED50 for this effect is 3-8 ng/mL.