Gene Name Synonym

IL-9, Interleukin-9, IL9, Cytokine P40

Species

Mouse

Accession

P15247

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

Gln19-Pro144 is expressed with a 10×His tag at the C terminus

Molecular Weight

15.6 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and   mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA   for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution,   it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier   protein). It is recommended to freeze aliquots at -20°C or -80°C for extended   storage.

BACKGROUND

Interleukin 9, also known as IL-9, is a cytokine (cell signaling   molecule) belonging to the group of interleukins. IL-9 is a   multifunctional cytokine secreted by TH2 lymphocytes. Interleukin-9   is reported to play important roles in innate and adaptive immunity in   extrarenal inflammatory diseases. The tolerogenic cytokine IL9 promotes   T regulatory cell function and allergic airway   inflammation. Eliminating endogenous IL9 enabled sensitization of   host T cells to tumors, leading to their early rejection without the   requirement of vaccines or immunomodulatory therapies. IL-9   predominantly produced by TH17 cells synergizes with TGF-β1   to differentiate naive CD4+ T cells into TH17 cells, while IL-9   secretion by TH17 cells is regulated by IL-23. IL-9 has been   demonstrated to be involved in several diseases, such as cancer,   autoimmunity and other pathogen-mediated immune-regulated diseases.

Data

SDS-PAGE

Bioactivity

Recombinant Mouse IL-9 Protein was determined by SDS-PAGE   with Coomassie Blue, showing a band at30-40 kDa.

Measured in a cell proliferation assay using MC/9 mouse mast cells. The   ED50 for this effect is 1-5 ng/mL.