Gene Name   Synonym

IL-9, Interleukin-9, IL9, Cytokine   P40

Species

Human

Accession

P15248

Source

HEK293   cells

SPECIFICATIONS

Amino acid   sequence

Met19-Ile144   is expressed with a 10×His tag at the C terminus

Molecular   Weight

17.6 kDa

Purity

>95.0%   as determined by SDS-PAGE

Endotoxin

<5 EU   mg^-1

Formulation

Lyophilized   from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants   before lyophilization

Reconstitution

Dissolved   in sterile water, it is recommended to add 0.1% HSA or BSA for long-term   preservation.

STORAGE

Stability and Storage

12 months   from date of receipt at -20°C to -70°C. After reconstitution, it is stable at   4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is   recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

Interleukin   9, also known as IL-9, is a cytokine (cell signaling molecule) belonging   to the group of interleukins. IL-9 is a multifunctional cytokine   secreted by TH2 lymphocytes. Interleukin-9 is reported to play   important roles in innate and adaptive immunity in extrarenal inflammatory   diseases. The tolerogenic cytokine IL9 promotes T regulatory   cell function and allergic airway inflammation. Eliminating   endogenous IL9 enabled sensitization of host T cells to tumors, leading   to their early rejection without the requirement of vaccines or immunomodulatory   therapies. IL-9 predominantly produced by TH17 cells synergizes with   TGF-β1 to differentiate naive CD4+ T cells into TH17 cells, while IL-9   secretion by TH17 cells is regulated by IL-23. IL-9 has been   demonstrated to be involved in several diseases, such as cancer,   autoimmunity and other pathogen-mediated immune-regulated diseases.

Data

SDS-PAGE

Bioactivity

Active   Recombinant Human IL-9 Protein was determined by SDS-PAGE with Coomassie   Blue, showing a band at 30-40 kDa.

Measured   in a cell proliferation assay using TF-1 human erythroleukemic cells. The   ED50 for this effect is 1-5 ng/mL.