Gene Name Synonym

IL-6, IL6, Interleukin-6, BSF2, HSF, IFNB2

Species

Human

Accession

P05231

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

Val30-Met212 is expressed with a 10×His tag at the N terminus

Molecular Weight

22.2 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU·mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and   mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA   for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution,   it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier   protein). It is recommended to freeze aliquots at -20°C or -80°C for extended   storage.

BACKGROUND

Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that   regulates cell growth and differentiation of various tissues, which is known   particularly for its role in the immune response and acute phase   reactions. IL6 is a potent inducer of the acute phase response. IL-6   protein is secreted by a variety of cell types including T cells and   macrophages as a phosphorylated and variably glycosylated molecule.IL-6   can lead to block the PI3K/AKT pathway. It exerts actions through its   heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain   and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130   (gp130) that binds the IL-6.

Data

SDS-PAGE

Bioactivity

Active Recombinant Human IL-6 Protein was determined by SDS-PAGE   with Coomassie Blue.

Measured in a cell proliferation assay using TF-1 human erythroleukemic   cells. The ED50 for this effect is 0.1-1 ng/mL.