Gene Name Synonym

IL-1 beta, IL1B, IL1F2, IL-1β

Species

Human

Accession

P01584

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

Ala117-Ser269 is expressed with a 10×His tag at the N terminus

Molecular Weight

18.8 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU·mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose and mannitol   are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA   for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution,   it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier   protein). It is recommended to freeze aliquots at -20°C or -80°C for extended   storage.

BACKGROUND

Interleukin-1 beta (IL1 beta or IL1B) also known as catabolin, is   a member of the interleukin 1 cytokine family. In clinical, it has   been reported that Interleukin (IL)-1 may influence Th1 / Th2
  immune responsiveness and has been implicated in the establishment of a   successful pregnancy. IL1B initially discovered as the major   endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx   and activation, T-cell activation and cytokine production, B-cell   activation and antibody production, and fibroblast proliferation and   collagen production.Interleukin (IL)-1B is a pro-inflammatory cytokine whose   expression in primary tumours has been identified as a potential   biomarker for predicting breast cancer patients at increased risk for   developing bone metastasis.

Data

SDS-PAGE

Bioactivity

Active Recombinant Human IL-1 beta Protein was determined by   SDS-PAGE with Coomassie Blue.

Measured in a cell proliferation assay using TF-1 human erythroleukemic   cells. The ED50 for this effect is 1-5 ng/mL.