Synonym

IL-1 alpha, Il1a, Hematopoietin-1, LEM, LAF, Pro interleukin 1 alpha,   IL1F1, Preinterleukin 1 alpha

Species

Mouse

Accession

P01582

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

Met115-Ser270 is expressed with a 10×His tag at the C terminus

Molecular Weight

19.4 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU·mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and   mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA   for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution,   it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier   protein). It is recommended to freeze aliquots at -20°C or -80°C for extended   storage.

BACKGROUND

IL-1 alpha is a member of the interleukin 1 cytokine family.   Located in the IL1 cluster, the IL1A gene is a   product of IL1B gene duplication, and consequently IL-1α and IL-1β   share a receptor, IL-1R1, and its downstream signaling. IL-1α has additional   functions, represented by the presence of a nuclear localization signal   within its pro-domain. IL-1 alpha is a pleiotropic cytokine involved in   various immune responses, inflammatory processes, and hematopoiesis which   is proteolytically processed and released in response to cell injury,   and thus induces apoptosis. IL-1α is a substrate of   noncanonical inflammatory caspases. IL-1 alpha stimulates thymocyte   proliferation by inducing IL-2 release, B-cell maturation and   proliferation, and fibroblast growth factor activity.

Data

SDS-PAGE

Bioactivity

Testing in progress

Active Recombinant Mouse IL-1 alpha Protein was determined by SDS-PAGE   with Coomassie Blue.