Synonym

IL-1   alpha, Il1a, Hematopoietin-1, LEM, LAF, Pro interleukin 1 alpha, IL1F1,   Preinterleukin 1 alpha

Species

Human

Accession

P01583

Source

CHO   cells

SPECIFICATIONS

Amino   acid sequence

Ser113-Ala271   is expressed with a 10×His tag at the C terminus

Molecular   Weight

19.4   kDa

Purity

>95.0%   as determined by SDS-PAGE

Endotoxin

<5 EU·mg-1

Formulation

Lyophilized   from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as   protectants before lyophilization.

Reconstitution

Dissolved   in sterile water, it is recommended to add 0.1% HSA or BSA for long-term   preservation.

STORAGE

Stability and Storage

12   months from date of receipt at -20°C to -70°C. After reconstitution, it is   stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein).   It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

IL-1   alpha is a member of the interleukin 1 cytokine family. Located in   the IL1 cluster, the IL1A gene is a product   of IL1B gene duplication, and consequently IL-1α and IL-1β share a   receptor, IL-1R1, and its downstream signaling. IL-1α has additional   functions, represented by the presence of a nuclear localization signal   within its pro-domain. IL-1 alpha is a pleiotropic cytokine involved in   various immune responses, inflammatory processes, and hematopoiesis which   is proteolytically processed and released in response to cell injury,   and thus induces apoptosis. IL-1α is a substrate of   noncanonical inflammatory caspases. IL-1 alpha stimulates thymocyte   proliferation by inducing IL-2 release, B-cell maturation and   proliferation, and fibroblast growth factor activity.

Data

SDS-PAGE

Bioactivity

Active   Recombinant Human IL-1 alpha Protein was determined by SDS-PAGE with   Coomassie Blue.

Measured   in a cell proliferation assay using TF-1 human erythroleukemic cells. The   ED50 for this effect is 0.05-0.2 μg/mL.